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Schenk & Hildebrandt (SH) Medium with Vitamins | Community media recipe | xPlant Pro

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Community recipeUpdated Jul 20, 2026

Schenk & Hildebrandt (SH) Medium with Vitamins

Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response v…

Recipe breakdown

Components

Calcium chloride (CaCl₂): 151.00 mg/L
Potassium nitrate (KNO₃): 2500.00 mg/L
Magnesium sulfate (MgSO₄): 195.05 mg/L
Ammonium dihydrogen phosphate ((NH₄)H₂PO₄): 300.00 mg/L

Grower context

Recipe notes

Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response varies with species, genotype, explant, and culture conditions. Formulated for callus induction and cell suspension culture in both monocots and dicots. High potassium and phosphate relative to MS.

Preparation

Target pH 5.8 before autoclaving. myo-Inositol is unusually high at 1000 mg/L — an order of magnitude above MS — so check you have not substituted an MS vitamin stock. Total salts including vitamins: 4193.75 mg/L.

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Primary source

Schenk, R.U., Hildebrandt, A.C. (1972). Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Canadian Journal of Botany 50(1) 199-204

doi:10.1139/b72-026

Compiled from

Duchefa S0225 — Schenk & Hildebrandt basal salt medium

Factual formulation, independently normalized

View original source

A community reference

Shared recipes are grower-contributed records, not verified instructions or guaranteed outcomes. Review the details against your own SOPs, local rules, and safety requirements before use.

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