Community media recipes | xPlant

Murashige & Skoog (MS) Medium with Vitamins

Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response varies with species, genotype, explant, and culture conditions.

Source: Duchefa M0222 — original MS concentration (1962)

18 components

Cobalt chloride hexahydrate (CoCl₂·6H₂O)Cupric sulfate pentahydrate (CuSO₄·5H₂O)Ferric sodium EDTA (FeNaEDTA)+15

Community recipe

Gamborg B5 Basal Salts

Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response varies with species, genotype, explant, and culture conditions.

Source: Duchefa G0209 — Gamborg B5 medium

13 components

Cobalt chloride hexahydrate (CoCl₂·6H₂O)Cupric sulfate pentahydrate (CuSO₄·5H₂O)Ferric sodium EDTA (FeNaEDTA)+10

Community recipe

McCown Woody Plant Medium (WPM) with Vitamins

Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response varies with species, genotype, explant, and culture conditions.

Source: Duchefa M0220 — McCown Woody Plant medium

17 components

Cupric sulfate pentahydrate (CuSO₄·5H₂O)Ferric sodium EDTA (FeNaEDTA)Boric acid (H₃BO₃)+14

Community recipe

Hosta Rooting Medium

Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response varies with species, genotype, explant, and culture conditions. Plantocol's reference formulation targets pH 5.7 and omits cytokinin.

Source: Plantocol — Hosta protocol

4 components

Murashige & Skoog basal mediumSucroseIndole-3-butyric acid (IBA)+1

Community recipe

Schenk & Hildebrandt (SH) Medium with Vitamins

Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response varies with species, genotype, explant, and culture conditions. Formulated for callus induction and cell suspension culture in both monocots and dicots. High potassium and phosphate relative to MS.

Source: Duchefa S0225 — Schenk & Hildebrandt basal salt medium

16 components

Calcium chloride (CaCl₂)Potassium nitrate (KNO₃)Magnesium sulfate (MgSO₄)+13

Community recipe

DKW/Juglans Medium with Vitamins

Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response varies with species, genotype, explant, and culture conditions. Developed for Juglans (walnut) rootstock and widely adopted for other woody species that respond poorly to MS.

Source: Duchefa D0247 — DKW/Juglans medium including vitamins

16 components

Calcium chloride (CaCl₂)Calcium nitrate dihydrate (Ca(NO₃)₂·2H₂O)Potassium dihydrogen phosphate (KH₂PO₄)+13

Community recipe

Nitsch & Nitsch Medium with Vitamins

Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response varies with species, genotype, explant, and culture conditions. The medium used to raise haploid Nicotiana plants from pollen grains; the standard starting point for anther and microspore culture.

Source: Duchefa N0224 — Nitsch medium including vitamins

18 components

Calcium chloride (CaCl₂)Potassium dihydrogen phosphate (KH₂PO₄)Potassium nitrate (KNO₃)+15

Community recipe

Anderson's Rhododendron Medium with Vitamins

Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response varies with species, genotype, explant, and culture conditions. Reduced-nitrogen formulation for ericaceous and other acid-loving woody plants — rhododendron, azalea, blueberry, raspberry.

Source: Duchefa A0202 — Anderson's Rhododendron including vitamins

16 components

Calcium chloride (CaCl₂)Potassium nitrate (KNO₃)Magnesium sulfate (MgSO₄)+13

Community recipe

Knudson C Orchid Basal Medium

Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response varies with species, genotype, explant, and culture conditions.

Source: Duchefa K0215 — Knudson C Orchid medium

8 components

Ferrous sulfate heptahydrate (FeSO₄·7H₂O)Manganese sulfate monohydrate (MnSO₄·H₂O)Calcium nitrate (Ca(NO₃)₂)+5

Community recipe

African Violet Initiation Medium

Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response varies with species, genotype, explant, and culture conditions. Plantocol's reference formulation targets pH 5.6–5.8.

Source: Plantocol — African Violet protocol

5 components

Murashige & Skoog basal mediumSucrose6-Benzylaminopurine (BAP)+2

Community recipe

African Violet Rooting Medium

Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response varies with species, genotype, explant, and culture conditions. Plantocol's reference formulation is hormone-free and targets pH 5.6–5.8.

Source: Plantocol — African Violet protocol

3 components

Murashige & Skoog basal mediumSucroseAgar

Community recipe

Monstera Establishment Medium

Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response varies with species, genotype, explant, and culture conditions. Plantocol's reference formulation targets pH 5.7.

Source: Plantocol — Monstera protocol

3 components

Murashige & Skoog basal mediumSucroseAgar

Community recipe

Hosta Initiation Medium

Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response varies with species, genotype, explant, and culture conditions. Plantocol's reference formulation targets pH 5.7.

Source: Plantocol — Hosta protocol

5 components

Murashige & Skoog basal mediumSucrose6-Benzylaminopurine (BAP)+2

Community recipe

Sarracenia leucophylla Germination Medium

Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response varies with species, genotype, explant, and culture conditions. Plantocol's reference formulation targets pH 4.5 and contains no plant growth regulators.

Source: Plantocol — Sarracenia protocol

3 components

Murashige & Skoog basal saltsSucrosePhytagel

Community recipe

Sarracenia oreophila Germination Medium

Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response varies with species, genotype, explant, and culture conditions. Plantocol's reference formulation targets pH 5.7 and contains no plant growth regulators. Confirm applicable conservation and collection rules before working with protected material.

Source: Plantocol — Sarracenia protocol

3 components

Murashige & Skoog basal saltsSucrosePhytagel

Media ideas worth passing around

Explore public recipes

Murashige & Skoog (MS) Medium with Vitamins

Gamborg B5 Basal Salts

McCown Woody Plant Medium (WPM) with Vitamins

Hosta Rooting Medium

Schenk & Hildebrandt (SH) Medium with Vitamins

DKW/Juglans Medium with Vitamins

Nitsch & Nitsch Medium with Vitamins

Anderson's Rhododendron Medium with Vitamins

Knudson C Orchid Basal Medium

African Violet Initiation Medium

African Violet Rooting Medium

Monstera Establishment Medium

Hosta Initiation Medium

Sarracenia leucophylla Germination Medium

Sarracenia oreophila Germination Medium

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