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Community Media Recipes

Explore plant tissue culture media recipes shared publicly by growers and laboratories. Compare formulations, ingredients, and notes before evaluating ideas for your own documented workflows.

A community reference Shared recipes are grower-contributed records, not verified instructions or guaranteed outcomes. Review the details against your own SOPs, local rules, and safety requirements before use.

15 public recipes

Community recipeUpdated Jul 20, 2026

Murashige & Skoog (MS) Medium with Vitamins

Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response varies with species, genotype, explant, and culture conditions.

Source: Duchefa M0222 — original MS concentration (1962)

18 ingredients
Cobalt chloride hexahydrate (CoCl₂·6H₂O)Cupric sulfate pentahydrate (CuSO₄·5H₂O)Ferric sodium EDTA (FeNaEDTA)+15 Show
  • Cobalt chloride hexahydrate (CoCl₂·6H₂O)0.025 mg/L
  • Cupric sulfate pentahydrate (CuSO₄·5H₂O)0.025 mg/L
  • Ferric sodium EDTA (FeNaEDTA)36.70 mg/L
  • Boric acid (H₃BO₃)6.20 mg/L
  • Potassium iodide (KI)0.83 mg/L
  • Manganese sulfate monohydrate (MnSO₄·H₂O)16.90 mg/L
  • Sodium molybdate dihydrate (Na₂MoO₄·2H₂O)0.25 mg/L
  • Zinc sulfate heptahydrate (ZnSO₄·7H₂O)8.60 mg/L
  • Calcium chloride (CaCl₂)332.02 mg/L
  • Potassium dihydrogen phosphate (KH₂PO₄)170.00 mg/L
  • Potassium nitrate (KNO₃)1900.00 mg/L
  • Magnesium sulfate (MgSO₄)180.54 mg/L
  • Ammonium nitrate (NH₄NO₃)1650.00 mg/L
  • Glycine2.00 mg/L
  • Myo-inositol100.00 mg/L
  • Nicotinic acid0.50 mg/L
  • Pyridoxine HCl0.50 mg/L
  • Thiamine HCl0.10 mg/L
Community recipeUpdated Jul 20, 2026

Gamborg B5 Basal Salts

Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response varies with species, genotype, explant, and culture conditions.

Source: Duchefa G0209 — Gamborg B5 medium

13 ingredients
Cobalt chloride hexahydrate (CoCl₂·6H₂O)Cupric sulfate pentahydrate (CuSO₄·5H₂O)Ferric sodium EDTA (FeNaEDTA)+10 Show
  • Cobalt chloride hexahydrate (CoCl₂·6H₂O)0.025 mg/L
  • Cupric sulfate pentahydrate (CuSO₄·5H₂O)0.025 mg/L
  • Ferric sodium EDTA (FeNaEDTA)36.70 mg/L
  • Boric acid (H₃BO₃)3.00 mg/L
  • Potassium iodide (KI)0.75 mg/L
  • Manganese sulfate monohydrate (MnSO₄·H₂O)10.00 mg/L
  • Sodium molybdate dihydrate (Na₂MoO₄·2H₂O)0.25 mg/L
  • Zinc sulfate heptahydrate (ZnSO₄·7H₂O)2.00 mg/L
  • Calcium chloride (CaCl₂)113.23 mg/L
  • Potassium nitrate (KNO₃)2500.00 mg/L
  • Magnesium sulfate (MgSO₄)121.56 mg/L
  • Sodium dihydrogen phosphate (NaH₂PO₄)130.44 mg/L
  • Ammonium sulfate ((NH₄)₂SO₄)134.00 mg/L
Community recipeUpdated Jul 20, 2026

McCown Woody Plant Medium (WPM) with Vitamins

Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response varies with species, genotype, explant, and culture conditions.

Source: Duchefa M0220 — McCown Woody Plant medium

17 ingredients
Cupric sulfate pentahydrate (CuSO₄·5H₂O)Ferric sodium EDTA (FeNaEDTA)Boric acid (H₃BO₃)+14 Show
  • Cupric sulfate pentahydrate (CuSO₄·5H₂O)0.25 mg/L
  • Ferric sodium EDTA (FeNaEDTA)36.70 mg/L
  • Boric acid (H₃BO₃)6.20 mg/L
  • Manganese sulfate monohydrate (MnSO₄·H₂O)22.30 mg/L
  • Sodium molybdate dihydrate (Na₂MoO₄·2H₂O)0.25 mg/L
  • Zinc sulfate heptahydrate (ZnSO₄·7H₂O)8.60 mg/L
  • Calcium chloride (CaCl₂)72.50 mg/L
  • Calcium nitrate tetrahydrate (Ca(NO₃)₂·4H₂O)471.26 mg/L
  • Potassium dihydrogen phosphate (KH₂PO₄)170.00 mg/L
  • Potassium sulfate (K₂SO₄)990.00 mg/L
  • Magnesium sulfate (MgSO₄)180.54 mg/L
  • Ammonium nitrate (NH₄NO₃)400.00 mg/L
  • Glycine2.00 mg/L
  • Myo-inositol100.00 mg/L
  • Nicotinic acid0.50 mg/L
  • Pyridoxine HCl0.50 mg/L
  • Thiamine HCl1.00 mg/L
Community recipeUpdated Jul 20, 2026

Hosta Rooting Medium

Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response varies with species, genotype, explant, and culture conditions. Plantocol's reference formulation targets pH 5.7 and omits cytokinin.

Source: Plantocol — Hosta protocol

4 ingredients
Murashige & Skoog basal mediumSucroseIndole-3-butyric acid (IBA)+1 Show
  • Murashige & Skoog basal medium1 ×
  • Sucrose20 g/L
  • Indole-3-butyric acid (IBA)1.0 mg/L
  • Agar8 g/L
Community recipeUpdated Jul 20, 2026

Schenk & Hildebrandt (SH) Medium with Vitamins

Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response varies with species, genotype, explant, and culture conditions. Formulated for callus induction and cell suspension culture in both monocots and dicots. High potassium and phosphate relative to MS.

Source: Duchefa S0225 — Schenk & Hildebrandt basal salt medium

16 ingredients
Calcium chloride (CaCl₂)Potassium nitrate (KNO₃)Magnesium sulfate (MgSO₄)+13 Show
  • Calcium chloride (CaCl₂)151.00 mg/L
  • Potassium nitrate (KNO₃)2500.00 mg/L
  • Magnesium sulfate (MgSO₄)195.05 mg/L
  • Ammonium dihydrogen phosphate ((NH₄)H₂PO₄)300.00 mg/L
  • Cobalt chloride hexahydrate (CoCl₂·6H₂O)0.10 mg/L
  • Cupric sulfate pentahydrate (CuSO₄·5H₂O)0.20 mg/L
  • Ferric sodium EDTA (FeNaEDTA)19.80 mg/L
  • Boric acid (H₃BO₃)5.00 mg/L
  • Potassium iodide (KI)1.00 mg/L
  • Manganese sulfate monohydrate (MnSO₄·H₂O)10.00 mg/L
  • Sodium molybdate dihydrate (Na₂MoO₄·2H₂O)0.10 mg/L
  • Zinc sulfate heptahydrate (ZnSO₄·7H₂O)1.00 mg/L
  • Myo-inositol1000.00 mg/L
  • Nicotinic acid5.00 mg/L
  • Pyridoxine HCl0.50 mg/L
  • Thiamine HCl5.00 mg/L
Community recipeUpdated Jul 20, 2026

DKW/Juglans Medium with Vitamins

Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response varies with species, genotype, explant, and culture conditions. Developed for Juglans (walnut) rootstock and widely adopted for other woody species that respond poorly to MS.

Source: Duchefa D0247 — DKW/Juglans medium including vitamins

16 ingredients
Calcium chloride (CaCl₂)Calcium nitrate dihydrate (Ca(NO₃)₂·2H₂O)Potassium dihydrogen phosphate (KH₂PO₄)+13 Show
  • Calcium chloride (CaCl₂)112.50 mg/L
  • Calcium nitrate dihydrate (Ca(NO₃)₂·2H₂O)1664.64 mg/L
  • Potassium dihydrogen phosphate (KH₂PO₄)265.00 mg/L
  • Potassium sulfate (K₂SO₄)1559.00 mg/L
  • Magnesium sulfate (MgSO₄)361.49 mg/L
  • Ammonium nitrate (NH₄NO₃)1416.00 mg/L
  • Cupric sulfate pentahydrate (CuSO₄·5H₂O)0.25 mg/L
  • Ferric sodium EDTA (FeNaEDTA)44.63 mg/L
  • Boric acid (H₃BO₃)4.80 mg/L
  • Manganese sulfate monohydrate (MnSO₄·H₂O)33.80 mg/L
  • Sodium molybdate dihydrate (Na₂MoO₄·2H₂O)0.39 mg/L
  • Zinc sulfate heptahydrate (ZnSO₄·7H₂O)17.00 mg/L
  • Glycine2.00 mg/L
  • Myo-inositol100.00 mg/L
  • Nicotinic acid1.00 mg/L
  • Thiamine HCl2.00 mg/L
Community recipeUpdated Jul 20, 2026

Nitsch & Nitsch Medium with Vitamins

Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response varies with species, genotype, explant, and culture conditions. The medium used to raise haploid Nicotiana plants from pollen grains; the standard starting point for anther and microspore culture.

Source: Duchefa N0224 — Nitsch medium including vitamins

18 ingredients
Calcium chloride (CaCl₂)Potassium dihydrogen phosphate (KH₂PO₄)Potassium nitrate (KNO₃)+15 Show
  • Calcium chloride (CaCl₂)166.00 mg/L
  • Potassium dihydrogen phosphate (KH₂PO₄)68.00 mg/L
  • Potassium nitrate (KNO₃)950.00 mg/L
  • Magnesium sulfate (MgSO₄)90.27 mg/L
  • Ammonium nitrate (NH₄NO₃)720.00 mg/L
  • Cupric sulfate pentahydrate (CuSO₄·5H₂O)0.025 mg/L
  • Ferric sodium EDTA (FeNaEDTA)36.70 mg/L
  • Boric acid (H₃BO₃)10.00 mg/L
  • Manganese sulfate monohydrate (MnSO₄·H₂O)18.94 mg/L
  • Sodium molybdate dihydrate (Na₂MoO₄·2H₂O)0.25 mg/L
  • Zinc sulfate heptahydrate (ZnSO₄·7H₂O)10.00 mg/L
  • Biotin0.05 mg/L
  • Folic acid0.50 mg/L
  • Glycine2.00 mg/L
  • Myo-inositol100.00 mg/L
  • Nicotinic acid5.00 mg/L
  • Pyridoxine HCl0.50 mg/L
  • Thiamine HCl0.50 mg/L
Community recipeUpdated Jul 20, 2026

Anderson's Rhododendron Medium with Vitamins

Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response varies with species, genotype, explant, and culture conditions. Reduced-nitrogen formulation for ericaceous and other acid-loving woody plants — rhododendron, azalea, blueberry, raspberry.

Source: Duchefa A0202 — Anderson's Rhododendron including vitamins

16 ingredients
Calcium chloride (CaCl₂)Potassium nitrate (KNO₃)Magnesium sulfate (MgSO₄)+13 Show
  • Calcium chloride (CaCl₂)332.02 mg/L
  • Potassium nitrate (KNO₃)480.00 mg/L
  • Magnesium sulfate (MgSO₄)180.54 mg/L
  • Sodium dihydrogen phosphate (NaH₂PO₄)330.60 mg/L
  • Ammonium nitrate (NH₄NO₃)400.00 mg/L
  • Cobalt chloride hexahydrate (CoCl₂·6H₂O)0.025 mg/L
  • Cupric sulfate pentahydrate (CuSO₄·5H₂O)0.025 mg/L
  • Ferric sodium EDTA (FeNaEDTA)73.40 mg/L
  • Boric acid (H₃BO₃)6.20 mg/L
  • Potassium iodide (KI)0.30 mg/L
  • Manganese sulfate monohydrate (MnSO₄·H₂O)16.90 mg/L
  • Sodium molybdate dihydrate (Na₂MoO₄·2H₂O)0.25 mg/L
  • Zinc sulfate heptahydrate (ZnSO₄·7H₂O)8.60 mg/L
  • Adenine sulphate80.00 mg/L
  • Myo-inositol100.00 mg/L
  • Thiamine HCl0.40 mg/L
Community recipeUpdated Jul 20, 2026

Knudson C Orchid Basal Medium

Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response varies with species, genotype, explant, and culture conditions.

Source: Duchefa K0215 — Knudson C Orchid medium

8 ingredients
Ferrous sulfate heptahydrate (FeSO₄·7H₂O)Manganese sulfate monohydrate (MnSO₄·H₂O)Calcium nitrate (Ca(NO₃)₂)+5 Show
  • Ferrous sulfate heptahydrate (FeSO₄·7H₂O)25.00 mg/L
  • Manganese sulfate monohydrate (MnSO₄·H₂O)5.68 mg/L
  • Calcium nitrate (Ca(NO₃)₂)241.30 mg/L
  • Potassium chloride (KCl)250.00 mg/L
  • Potassium dihydrogen phosphate (KH₂PO₄)250.00 mg/L
  • Magnesium sulfate (MgSO₄)122.15 mg/L
  • Ammonium nitrate (NH₄NO₃)500.00 mg/L
  • Ammonium sulfate ((NH₄)₂SO₄)500.00 mg/L
Community recipeUpdated Jul 20, 2026

African Violet Initiation Medium

Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response varies with species, genotype, explant, and culture conditions. Plantocol's reference formulation targets pH 5.6–5.8.

Source: Plantocol — African Violet protocol

5 ingredients
Murashige & Skoog basal mediumSucrose6-Benzylaminopurine (BAP)+2 Show
  • Murashige & Skoog basal medium1 ×
  • Sucrose30 g/L
  • 6-Benzylaminopurine (BAP)1.0 mg/L
  • Indole-3-acetic acid (IAA)0.1 mg/L
  • Agar8 g/L
Community recipeUpdated Jul 20, 2026

African Violet Rooting Medium

Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response varies with species, genotype, explant, and culture conditions. Plantocol's reference formulation is hormone-free and targets pH 5.6–5.8.

Source: Plantocol — African Violet protocol

3 ingredients
Murashige & Skoog basal mediumSucroseAgarShow
  • Murashige & Skoog basal medium2.22 g/L
  • Sucrose20 g/L
  • Agar8 g/L
Community recipeUpdated Jul 20, 2026

Monstera Establishment Medium

Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response varies with species, genotype, explant, and culture conditions. Plantocol's reference formulation targets pH 5.7.

Source: Plantocol — Monstera protocol

3 ingredients
Murashige & Skoog basal mediumSucroseAgarShow
  • Murashige & Skoog basal medium1 ×
  • Sucrose30 g/L
  • Agar7 g/L
Community recipeUpdated Jul 20, 2026

Hosta Initiation Medium

Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response varies with species, genotype, explant, and culture conditions. Plantocol's reference formulation targets pH 5.7.

Source: Plantocol — Hosta protocol

5 ingredients
Murashige & Skoog basal mediumSucrose6-Benzylaminopurine (BAP)+2 Show
  • Murashige & Skoog basal medium1 ×
  • Sucrose30 g/L
  • 6-Benzylaminopurine (BAP)2.0 mg/L
  • 1-Naphthaleneacetic acid (NAA)0.1 mg/L
  • Agar8 g/L
Community recipeUpdated Jul 20, 2026

Sarracenia leucophylla Germination Medium

Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response varies with species, genotype, explant, and culture conditions. Plantocol's reference formulation targets pH 4.5 and contains no plant growth regulators.

Source: Plantocol — Sarracenia protocol

3 ingredients
Murashige & Skoog basal saltsSucrosePhytagelShow
  • Murashige & Skoog basal salts1.48 g/L
  • Sucrose30 g/L
  • Phytagel4.5 g/L
Community recipeUpdated Jul 20, 2026

Sarracenia oreophila Germination Medium

Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response varies with species, genotype, explant, and culture conditions. Plantocol's reference formulation targets pH 5.7 and contains no plant growth regulators. Confirm applicable conservation and collection rules before working with protected material.

Source: Plantocol — Sarracenia protocol

3 ingredients
Murashige & Skoog basal saltsSucrosePhytagelShow
  • Murashige & Skoog basal salts0.74 g/L
  • Sucrose30 g/L
  • Phytagel4.5 g/L

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