Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response v…
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Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response varies with species, genotype, explant, and culture conditions. The medium used to raise haploid Nicotiana plants from pollen grains; the standard starting point for anther and microspore culture.
Target pH 5.8 before autoclaving. Biotin and folic acid are heat-labile — filter-sterilise them (0.22 µm) and add to cooled medium below about 50 °C rather than autoclaving them. Total salts including vitamins: 2178.74 mg/L.
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