Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response v…
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Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response varies with species, genotype, explant, and culture conditions. Reduced-nitrogen formulation for ericaceous and other acid-loving woody plants — rhododendron, azalea, blueberry, raspberry.
Target pH 4.5-5.0 before autoclaving; this medium is deliberately acidic for ericaceous species. Nitrate is roughly a quarter of MS strength, which is the point of the formulation — do not 'correct' it upward. Contains adenine sulphate at 80 mg/L, which acts as a weak cytokinin, so reduce added cytokinin accordingly. Total salts including vitamins: 2009.26 mg/L.
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