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Bibliothèque de milieux communautaire

Des idées de milieux qui méritent d’être partagées

Explorez les recettes que des cultivateurs ont choisi de rendre publiques. Comparez les composants et les notes avant de décider ce qui convient à votre propre flux documenté.

Nouveau dans la communauté

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Chaque recette a été explicitement partagée par son propriétaire. Ouvrez-la pour consulter ses composants, ses notes et sa conversation.

Recette communautaire

Murashige & Skoog (MS) Medium with Vitamins

Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response varies with species, genotype, explant, and culture conditions.

Source: Duchefa M0222 — original MS concentration (1962)

18 composants
Cobalt chloride hexahydrate (CoCl₂·6H₂O)Cupric sulfate pentahydrate (CuSO₄·5H₂O)Ferric sodium EDTA (FeNaEDTA)+15
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Recette communautaire

Gamborg B5 Basal Salts

Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response varies with species, genotype, explant, and culture conditions.

Source: Duchefa G0209 — Gamborg B5 medium

13 composants
Cobalt chloride hexahydrate (CoCl₂·6H₂O)Cupric sulfate pentahydrate (CuSO₄·5H₂O)Ferric sodium EDTA (FeNaEDTA)+10
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Recette communautaire

McCown Woody Plant Medium (WPM) with Vitamins

Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response varies with species, genotype, explant, and culture conditions.

Source: Duchefa M0220 — McCown Woody Plant medium

17 composants
Cupric sulfate pentahydrate (CuSO₄·5H₂O)Ferric sodium EDTA (FeNaEDTA)Boric acid (H₃BO₃)+14
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Recette communautaire

Hosta Rooting Medium

Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response varies with species, genotype, explant, and culture conditions. Plantocol's reference formulation targets pH 5.7 and omits cytokinin.

Source: Plantocol — Hosta protocol

4 composants
Murashige & Skoog basal mediumSucroseIndole-3-butyric acid (IBA)+1
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Recette communautaire

Schenk & Hildebrandt (SH) Medium with Vitamins

Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response varies with species, genotype, explant, and culture conditions. Formulated for callus induction and cell suspension culture in both monocots and dicots. High potassium and phosphate relative to MS.

Source: Duchefa S0225 — Schenk & Hildebrandt basal salt medium

16 composants
Calcium chloride (CaCl₂)Potassium nitrate (KNO₃)Magnesium sulfate (MgSO₄)+13
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Recette communautaire

DKW/Juglans Medium with Vitamins

Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response varies with species, genotype, explant, and culture conditions. Developed for Juglans (walnut) rootstock and widely adopted for other woody species that respond poorly to MS.

Source: Duchefa D0247 — DKW/Juglans medium including vitamins

16 composants
Calcium chloride (CaCl₂)Calcium nitrate dihydrate (Ca(NO₃)₂·2H₂O)Potassium dihydrogen phosphate (KH₂PO₄)+13
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Recette communautaire

Nitsch & Nitsch Medium with Vitamins

Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response varies with species, genotype, explant, and culture conditions. The medium used to raise haploid Nicotiana plants from pollen grains; the standard starting point for anther and microspore culture.

Source: Duchefa N0224 — Nitsch medium including vitamins

18 composants
Calcium chloride (CaCl₂)Potassium dihydrogen phosphate (KH₂PO₄)Potassium nitrate (KNO₃)+15
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Recette communautaire

Anderson's Rhododendron Medium with Vitamins

Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response varies with species, genotype, explant, and culture conditions. Reduced-nitrogen formulation for ericaceous and other acid-loving woody plants — rhododendron, azalea, blueberry, raspberry.

Source: Duchefa A0202 — Anderson's Rhododendron including vitamins

16 composants
Calcium chloride (CaCl₂)Potassium nitrate (KNO₃)Magnesium sulfate (MgSO₄)+13
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Recette communautaire

Knudson C Orchid Basal Medium

Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response varies with species, genotype, explant, and culture conditions.

Source: Duchefa K0215 — Knudson C Orchid medium

8 composants
Ferrous sulfate heptahydrate (FeSO₄·7H₂O)Manganese sulfate monohydrate (MnSO₄·H₂O)Calcium nitrate (Ca(NO₃)₂)+5
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Recette communautaire

African Violet Initiation Medium

Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response varies with species, genotype, explant, and culture conditions. Plantocol's reference formulation targets pH 5.6–5.8.

Source: Plantocol — African Violet protocol

5 composants
Murashige & Skoog basal mediumSucrose6-Benzylaminopurine (BAP)+2
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Recette communautaire

African Violet Rooting Medium

Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response varies with species, genotype, explant, and culture conditions. Plantocol's reference formulation is hormone-free and targets pH 5.6–5.8.

Source: Plantocol — African Violet protocol

3 composants
Murashige & Skoog basal mediumSucroseAgar
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Recette communautaire

Monstera Establishment Medium

Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response varies with species, genotype, explant, and culture conditions. Plantocol's reference formulation targets pH 5.7.

Source: Plantocol — Monstera protocol

3 composants
Murashige & Skoog basal mediumSucroseAgar
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Recette communautaire

Hosta Initiation Medium

Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response varies with species, genotype, explant, and culture conditions. Plantocol's reference formulation targets pH 5.7.

Source: Plantocol — Hosta protocol

5 composants
Murashige & Skoog basal mediumSucrose6-Benzylaminopurine (BAP)+2
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Recette communautaire

Sarracenia leucophylla Germination Medium

Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response varies with species, genotype, explant, and culture conditions. Plantocol's reference formulation targets pH 4.5 and contains no plant growth regulators.

Source: Plantocol — Sarracenia protocol

3 composants
Murashige & Skoog basal saltsSucrosePhytagel
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Recette communautaire

Sarracenia oreophila Germination Medium

Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response varies with species, genotype, explant, and culture conditions. Plantocol's reference formulation targets pH 5.7 and contains no plant growth regulators. Confirm applicable conservation and collection rules before working with protected material.

Source: Plantocol — Sarracenia protocol

3 composants
Murashige & Skoog basal saltsSucrosePhytagel
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