Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response v…
Contexte du cultivateur
Reference formulation per litre. Verify reagent hydration states, calculations, and your lab's validated preparation method before use. Plant response varies with species, genotype, explant, and culture conditions. Developed for Juglans (walnut) rootstock and widely adopted for other woody species that respond poorly to MS.
Target pH 5.5 before autoclaving. Calcium nitrate is the dominant macro salt here rather than ammonium nitrate; prepare it in its own stock, because concentrated calcium and sulfate/phosphate stocks precipitate on contact. Total salts including vitamins: 5584.50 mg/L.
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