2,4-D (2,4-Dichlorophenoxyacetic acid) — 1 g

The most potent synthetic auxin for callus induction and somatic embryogenesis. At 0.5–5.0 mg/L, 2,4-D dedifferentiates plant tissues to produce highly proliferative, often embryogenic callus — the primary growth regulator in cereal transformation protocols (maize, wheat, barley). Dissolve in dilute NaOH or ethanol.